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Primary antibodies to DPEP-1 (Proteintech, #12222-1-AP, 1:1000), 4-HNE (Abcam, #ab46545, 1:1000), FTH1 (Abcam, #ab65080, 1:1000), GCLM (Abcam, #EPR6667, 1:1000), GCLC (Abcam, #EP13475, 1:2000), ZBP1 (Adipogen, #AG-20B-0010-c100, 1:1000), FSP1 (Santa Cruz, #sc-377120, 1:5000), and -actin (Cell Signaling, #3700S, 1:500) were diluted in skimmed milk (Carl Roth, #T145.2)

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Lung adenocarcinoma of never smokers and smokers harbor differential regions of genetic alteration and exhibit different levels of genomic instability